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. 2022 Oct 19;15:1020803. doi: 10.3389/fnmol.2022.1020803

FIGURE 3.

FIGURE 3

Co-transduction of dual-AAV-ie vectors in the damaged adult mouse utricle. AAV-ie-CAG-EGFP and AAV-ie-CAG-mCherry were injected into the inner ear of adult Pou4f3+/DTR mice 10 days following diphtheria toxin administration. Utricles were sampled 2 weeks after the surgery. (A–A”’) Low-magnification images show extensive expression of GFP and mCherry throughout the utricle, with the loss of most hair cells (HCs). (B–C”’) High-magnification images show co-transduction of dual-AAV-ie-CAG vectors in residual HCs (B–B”’; arrowheads; representative images of the extrastriolar region) and abundant supporting cells (SCs) (C–C”’; arrows; representative images of the extrastriolar region). Scale bars, 50 μm in A for (A–A”’); 20 μm in B for (B–C”’). (D,E) Comparative analysis of the co-transduction rates of HCs (D) and SCs (E) in damaged and normal adult mice. The co-transduction rates of HCs in damaged adult mice are significantly higher than those of normal mice in striolar and extrastriolar regions, whereas the co-transduction rates of SCs are comparable. Data are mean ± SEM. P-values were calculated using Student’s t-test. “ns,” not significant. **P < 0.01.