Fig. 3.
PC titration and drug tolerance testing for ADA affinity assay. (A) Affinity curves derived from titrated PC, spiked in neat serum at 1667–6.86 ng/mL and processed through the ACE and affinity assay workflow. The free ADA LBA signal window must be high enough to demonstrate a sufficient level of inhibition over the range of drug concentrations so KD may be accurately calculated. Asterisks denote that the 6.86 ng/mL spiked PC affinity curve was the only PC concentration to fail the inhibition “flatness” test criteria; all other spike levels (1667–20.6 ng/mL) passed; (B) PC affinity curves with and without PF-06480605, 25 μg/mL