Figure 2.

Effect of Fer-1 on osteoblasts treated with HG. (a) JC-1 staining showed a decreased level of the mitochondrial membrane potential in osteoblasts under HG conditions. Scale bar = 20 μm. (b) Flow cytometry analysis of intracellular ROS generation. (c) Lipid peroxidation was detected using the MDA assay kits. (d) Lipid peroxidation was detected by the 4-HNE assay kits. (e) Fer-1 reversed the decrease of osteoblast viability induced by HG, measured by CCK-8 assay. (f) The amount of cell injury was evaluated by LDH release assay. (g) Detection of lipid peroxidation by C11 BODIPY 581/591 fluorescent probe. Scale bar = 20 μm. All data are presented as the mean ± SD of three independent experiments. ^∗P < 0.05 vs. NG and ^#P < 0.05 vs. HG.