FIG. 2.

Both CD4⁺ and CD8⁺ T cells lose L-selectin in response to cryptococcal antigen. (A and B) Profiles of L-selectin-stained CD4⁺ and CD8⁺ cells, respectively, from one representative individual of the 10 individuals whose PBMC were analyzed after a 2-h incubation in medium alone (open areas). and after a 2-h treatment with CneF (shaded areas). (C) Mean percentage of L-selectin positive cells ± SEM and the mean fluorescence intensity ± SEM of L-selectin staining in the CD4⁺ and CD8⁺ cell populations which did not completely lose L-selectin after a 2-h treatment with medium alone (untreated) and after a 2-h treatment with CneF. P values from a paired t test for the comparisons of the CneF-treated cells with the untreated cells are also shown.