TABLE 2.

Expression of various T-cell surface molecules on two T-cell lines after incubation with various reagents^a

Reagent	Mean fluorescence intensity
	Anti-CD28		Anti-CD45RA		Anti-CD2		Anti-CD18		Anti-L-selectin
	Ju	JR	Ju	JR	Ju	JR	Ju	JR	Ju	JR
Medium	15	0	28	19	43	43	21	20	100	93
PMA	13	0	29	21	51	51	24	19	17	13
GXM	16	0	28	21	47	51	21	20	41	70
GalXM	16	0	28	21	44	45	21	22	87	87
MP	15	0	28	21	44	44	21	20	97	87

^aJu, Jurkat; JR, J.RT3-T3.5. The T cells (10⁶) were incubated for 2 h at 37°C with 100 ng of PMA/ml or 1 mg of GXM, GalXM, or MP/ml in serum-free RPMI 1640 medium. CD28, CD45RA, CD2, CD18, or L-selectin expression on the two T-cell lines was determined by immunofluorescence staining with the designated antibody and subjecting the cells to flow cytometric analysis. The data are from one experiment representative of the two experiments performed.