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. 2022 Jun 17;71(11):2256–2271. doi: 10.2337/db22-0260

Figure 7.

Figure 7

BCATm is responsible for multiple-integrated metabolism in IRF4-deleted skeletal muscle. A: Cellular BCAA level of C2C12 myoblasts (n = 4) (*P < 0.05 and ***P < 0.001). B: Western blot analysis of PTG expression in C2C12 cells. Protein amount was quantified with ImageJ (n = 3) (*P < 0.05). C: Cellular glycogen level of C2C12 myoblasts (n = 4) (*P < 0.05). D and E: BCAA and glycogen levels in GAS of male MKO+AAV-BCATm mice, MKO+AAV-GFP mice, and Flox+AAV-GFP mice (n = 5–7) (*P < 0.05, **P < 0.01). F: Quantitative PCR analysis of FAO genes (n = 5) (*P < 0.05). G: Western blot analysis of the expression of PGC-1α. Protein amount was quantified with ImageJ (n = 4) (*P < 0.05). H: Western blot analysis of the expression of BCATm in muscle of humans with or without obesity. Protein amount was quantified with ImageJ (n = 5) (***P < 0.001). I: Working model. All results are expressed as means ± SEM. AU, arbitrary units; Mito, mitochondrion.