Figure 2.

Role of miR-130b/301b in beige adipogenic differentiation of progenitor cells isolated from fat tissue. SVF cells were isolated from iWAT from WT mice at the time of weaning and transfected with miR-130b-3p mimics, miR-301-3p mimics, or negative control. Beige adipogenic differentiation was induced at 2 days after transfection. At 6 days after differentiation, oil red O staining of beige differentiated cells (A), protein abundance of UCP1 (B), and Ucp1, Cox8a, Pgc-1α, Dio2, Adiponectin, PPARγ2, and Perilipin A mRNAs (C) are shown. Con, control. Mean ± SD, n = 3–4 in each group. *P < 0.05, **P < 0.01, ***P < 0.001 compared with negative control (−).