Figure 3.

Role of miR-130b/301b in mitochondrial respiration in beige adipogenic differentiated progenitor cells isolated from fat tissues. SVF cells were isolated from iWAT from WT mice at the time of weaning. The progenitor cells were transfected with miR-130b-3p mimics, miR-301-3p mimics, or negative control (Con). Beige adipogenic differentiation was induced at 2 days after transfection. At 3 days following beige differentiation, OCR was measured over time and after stress treatment of Oligomycin (Oligo, 1 μmol/L), FCCP (1 μmol/L), and rotenone and antimycin A (R/A) (0.5 μmol/L). Indices of baseline OCR, proton leak, maximal respiration, and ATP production were calculated according to altered OCR following the treatment. Mean ± SD, n = 8 in each group. *P < 0.05; **P < 0.01; ***P < 0.001.