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. 2022 Aug 31;611(7934):139–147. doi: 10.1038/s41586-022-05273-0

Fig. 3. IgG1 clonotypes are both antiviral and autoreactive.

Fig. 3

a, Overview of clonotype (mAb) testing from patients ICU-1 and ICU-2 (total n = 107). Clonotypes were selected from the IgG1+ low-selection compartment described in Figs. 1,2. Left: heatmaps of mAb (rows) binding to indicated antigens (columns). Middle: KD, antibody affinities confirmed through HT-SPR; IGHV4-34, clonotype encodes IGHV4-34 receptor; germline, clonotype shows germline heavy and light chain configurations; autoreactive, clonotype shows autoreactivity against indicated autoantigen. Right: Ab designation to aid tracking throughout Fig. 4. b, SARS-CoV-2 antigen targeting across all 107 mAbs. c, MFI measurements of Hep2 cell line reactivity by synthesized mABs using immunofluorescence. Selected mAb designations are indicated (Fig. 4a). d, Anti-GBM ELISA testing of isolated mAbs (optical density, OD). Selected clonotype designations are indicated (Fig. 4a). In c and d, summary statistics are mean negative test value ±3 s.d. pos., positive; neg., negative.