Extended Data Fig. 2. IgM-connected IgG1 ASC expansion in severe/critical COVID-19.

(a–c) ASCs from HD (n = 3), OUT-C (n = 4), or ICU-C (n = 6) patient cohorts were sorted for single B cell repertoire sequencing and subsequent analysis. (a) Isotype frequencies of individual patients within the ICU-C, OUT-C, and HD cohorts (b) ASC subclass frequencies by indicated isotype in HD, OUT-C, and ICU-C cohorts. (c) Clonotype connectivity between IgM and IgG1 ASCs in HD, OUT-C and ICU-C cohorts. (b, c) Statistical significance was determined using ANOVA with Tukey’s multiple-comparisons testing between all groups. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.