Extended Data Fig. 9. Characterization of IFN-γ-mediated lesions in the white matter of young and old mice.
a, Representative confocal images of IFN-γ-mediated lesions in young and old mice and of the vehicle control stained for STAT1 (white) and DAPI (nuclei, blue). b, The data shown in Fig. 5 for IFN-γ-mediated lesions in 18-months old mice is shown here in comparison to vehicle control injections in 18-months old mice. Representative confocal images and quantifications of IFN-γ-mediated lesions in old mice and of the vehicle control showing MBP. The intensity of the staining for MBP was used to quantify the extent of demyelination (n = 3 mice per group, **P = 0.0040; data are means±s.e.m.; P value represents a two-sided Student’s t-test). c, Representative confocal images of IFN-γ-mediated lesions in old mice showing MAC2+ cells (red) with myelin (green) stained by FluoroMyelin. All scale bars, 20 μm. The experiments were repeated three times independently with similar results.