Table 3.
Serum analysis
| Variable | Male offspring | Female offspring | ||||||
|---|---|---|---|---|---|---|---|---|
| Con (n=11 or 12) |
Ob (n=6–8) |
Ob-Met (n=9–11) |
p value (ANOVA) |
Con (n=10–12) |
Ob (n=11 or 12) |
Ob-Met (n=10 or 11) |
p value (ANOVA) |
|
| Glucose (mmol/l)a | 6.9±0.3 | 7.6±0.4 | 6.8±0.2 | NS | 6.6±0.2 | 6.5±0.2 | 6.5±0.2 | NS |
| Insulin (pmol/l)b | 146±14 | 198±28 | 217±41 | NS | 101±16 | 134±17 | 180±23* | 0.0227 |
| Cholesterol (mmol/l)b,c | 3.1±0.1 | 3.4±0.2 | 3.6±0.3 | NS | 2.1±0.1 | 2.0±0.1 | 2.0±0.1 | NS |
| Triglycerides (mmol/l)b | 1.09±0.06 | 0.99±0.07 | 1.09±0.06 | NS | 0.97±0.05 | 1.06±0.06 | 1.12±0.05 | NS |
| NEFA (mmol/l) | 1.60±0.06 | 1.64±0.12 | 1.61±0.07 | NS | 1.35±0.05 | 1.28±0.05 | 1.31±0.05 | NS |
| Leptin (ng/ml)d | 16.6±2.8 | 34.8±4.5* | 33.7±5.5* | <0.001 | 23.7±2.5 | 27.9±3.2 | 49.3±5.8***,† | <0.001 |
All data reflect serum from 16 h fasted mice at 12 months of age and are represented as mean ± SEM
Outliers were excluded (Grubb’s method) as follows: from male Con for insulin (396 pmol/l); from male Ob for cholesterol (5.5 mmol/l); from female Con for glucose (excluded value 9.7 mmol/l), insulin (337 pmol/l) and NEFA (2.33 mmol/l); from female Ob for NEFA (2.22 mmol/l); and from female Ob-Met for glucose (3.0 mmol/l) and for NEFA (2.32 mmol/l)
aSerum was not collected for one male Ob mouse therefore n=8 for glucose compared with n=7 for other variables
bOne sample from a male Ob-Met mouse was not analysed for cholesterol, triglycerides or insulin
cCholesterol was not detectable for one male control mouse and female control mouse (<1.3 mmol/l) and therefore these data were excluded from analysis
dOne sample from a female Ob mouse was not analysed for leptin
*p<0.05 and ***p<0.001 vs Con offspring; †p<0.01 vs Ob offspring (one-way ANOVA with Tukey’s multiple comparison test)