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. 2022 Nov 2;12:18529. doi: 10.1038/s41598-022-23304-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the expression vector containing targeted gene used in this study and confirmation of VEGF gene by PCR and final approval of cloning by BamHI restriction enzyme. (A) pcDNA 3.1 (-) expression vector containing VEGF fragment downstream of the CMV promoter. (B) Agarose gel electrophoresis revealed that the PCR amplified target gene fragment was in the expected position, 620 bp of VEGF. (C) Cloning confirmation was done from the intermediate vector in the expression vector via BamHI restriction enzyme. Column 1: pcDNA 3.1 (-) containing VEGF vector cut with BamHI enzyme that cut fragment is 620 bp Column 2: The uncut pcDNA 3.1 (-) containing VEGF vector. Column 3: 1 Kb molecular weight size marker (Thermos Fisher Scientifics, USA).