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. 2022 Nov 2;12:18529. doi: 10.1038/s41598-022-23304-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transfection efficiency and investigating overexpression of VEGF gene in manipulated Hu02. (A) Fluorescent image of transfected Hu02 cells expressing EGFP (green fluorescence) as a transfection control. (B) Fold change of VEGF mRNA expression in manipulated Hu02 compared to untransfected Hu02 was quantified using qRT-PCR. B-actin was used as an internal control. (C) Western blotting analysis of VEGF overexpression among (1) control, (2) transfected, and (3) untransfected groups. (***P ≤ 0.001).