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. 2022 Oct 20;13:943627. doi: 10.3389/fphar.2022.943627

FIGURE 2.

FIGURE 2

B-27 encourages SH-SY5Y cells were seeded into 6-well plates at a density of 300,000 cells per well in DMEM/F-12 + 10% FBS. Once SH-SY5Y cells reached approximately 70% confluency, media was replaced with either: DMEM/F-12 only, DMEM/F-12 + 10% FBS, DMEM/F-12 + 2% B-27 supplement or DMEM/F-12 + 5% B-27 supplement. Cells were imaged using the EVOS FLoid microscope (Thermo Scientific) before media was replaced and 48 h after media was replaced. Scale bar = 100 μm. DMEM/F-12, Dulbecco’s modified eagle medium/nutrient mixture F-12 with GlutaMAX supplement; FBS, fetal bovine serum.