Skip to main content
. 2022 Oct 20;13:943627. doi: 10.3389/fphar.2022.943627

FIGURE 6.

FIGURE 6

B-27 induces SH-SY5Y cell differentiation. SH-SY5Y cells were cultured in DMEM/F-12 only, or DMEM/F-12 supplemented with either 10% FBS, 2% B-27 or 5% B-27 for (A) 48 h or (B) 96 h. qRT-PCR was performed to measure mRNA expression of differentiation markers, GAP43, TUBB3 and SYP. Relative expression of GAP43, TUBB3, and SYP (standardized to GAPDH and POLR2A) normalized to the DMEM/F-12 + 10% FBS culture conditions. Data presented as mean ± SEM. N = 3 independent biological samples with two to three technical replicates each. Statistical significance against the DMEM/F-12 + 10% FBS condition was determined using ordinary one-way ANOVA and Tukey’s multiple comparison tests in GraphPad Prism version 9.0.0 for Mac, GraphPad Software, San Diego, California, United States, www.graphpad.com. ns = not significant; *p < 0.05; **p < 0.01; ***p < 1 × 10–3; ****p < 1 × 10–4. DMEM/F-12, Dulbecco’s modified eagle medium/nutrient mixture F-12 with GlutaMAX supplement; FBS, fetal bovine serum; GAP43, Growth associated protein 43; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; mRNA, messenger RNA; POLR2A, RNA polymerase II subunit A; SEM, standard error of the mean; SYP, Synaptophysin; TUBB3, Tubulin beta 3 class III.