TABLE 1.
L. monocytogenes straina | Amino acid sequenceb
|
|
---|---|---|
LLO 91–99 | p60 217–225 | |
Wild type | GYKDGNEYI | KYGVSVQDI |
p60 218F | GYKDGNEYI | KFGVSVQDV |
92F | GFKDGNEYI | KYGVSVQDI |
The L. monocytogenes strains utilized for study include the wild type; the 92F mutant, which contains a Y-to-F substitution at aa 92 of LLO; or p60 218F, which contains a Y-to-F substitution at aa 218 of p60 and an I-to-V substitution at aa 225. (Substitutions are underlined.)
The amino acid sequence is given in the single-letter code for each of the strains listed. The amino acid sequence was determined by DNA sequencing as described in Materials and Methods.