FIGURE 1.
Evaluation of resveratrol-βcd anti-tumor effects in vitro. (A) CCK-8 assessment of the inhibitory effects of resveratrol-βcd or resveratrol or an equal volume of cyclodextrin on cell proliferation at the indicated times and concentrations. The heatmap values are the average values of three replicates. (B) Typical diagram of apoptosis flow cytometry after treatment of LLC cells with 10 μM resveratrol-βcd at different time points. (C) Statistical results of apoptosis at the indicated times. Error bar = mean ± S.D. (D) Typical flow cytometry diagram of LLC cell senescence analysis after treatment with 10 μM resveratrol-βcd for the indicated times. p16 was used as the marker of senescence. (E) Statistical results of the senescence analysis. Error bar = mean ± S.D. (F) Typical β-gal graph of LLC cells after treatment with 10 μM resveratrol-βcd at the indicated times. Cyan-stained cells were considered positive for senescence. Scale bar = 50 μm; positive cells per 103 cells were counted. (G) Statistical results. Error bar = mean ± S.D. (H) Time course of mouse body weight after treatment with the indicated concentrations of resveratrol-βcd. Error bar = mean ± S.D., n = 5.