Fig. 2.

Effect on barrier properties investigated by TEER measurements after incubation with different stimuli. The Caco-2 cells stimulated with the supernatant of LPS-stimulated MDM resulted in a significant decrease of TEER to 38% of the initial value while the stimulation with non-stimulated MDM supernatant showed only a slight decrease. Results are represented as mean ± SD for in summary n = 9 wells for each group performed in 3 biological replicates (A). In the co-culture system, stable TEER values could be obtained for the combination of Caco-2 and MDM without any stimulation (= medium control), which indicates an intact epithelial barrier. The LPS-stimulation of the MDM in the basolateral compartment induced a significant decrease in TEER. Results are represented as mean ± SD and for in summary n = 18 wells for each group performed in three biological replicates (B). *p < 0.05, **p < 0.01, and ***p < 0.001 indicate significant difference