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. 2022 Nov 3;1(4-5):365–383. doi: 10.1007/s44164-022-00035-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Assessment of in vitro drug efficacy for the readouts TEER, TNF-α, IL-6, and IL-8. The drug efficacy was calculated in percent relative to the medium control (healthy, 100% drug efficacy) and the LPS control (inflamed, 0% drug efficacy) against the severity level represented by the respective drugs. The readouts TEER, TNF-α, IL-6, and IL-8 were used for the evaluation of drug efficacy with the co-culture model. Results are represented as mean ± SD for in summary n = 10 wells for each group performed in five biological replicates for the TEER measurements and for in summary n = 6 wells for each group performed in three biological replicates for the cytokines