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. Author manuscript; available in PMC: 2023 Oct 27.
Published in final edited form as: J Med Chem. 2022 Oct 13;65(20):13714–13735. doi: 10.1021/acs.jmedchem.2c00864

Table 1.

Hits Using Protein-Detected NMR Fragment Screening of PBRM1-BD2

Compound Structure PBRM1-BD2 NMR Kd (μM) LEb PBRM1-BD2 ΔTm (°C)a
5 graphic file with name nihms-1843423-t0010.jpg 45.3 ± 8.1 0.31 1.0 ± 0.2
6 graphic file with name nihms-1843423-t0011.jpg 79 ± 35 0.33 1.5 ± 1.0
6a graphic file with name nihms-1843423-t0012.jpg 170 ± 35 0.4 1.0 ± 0.1
6b graphic file with name nihms-1843423-t0013.jpg 902 ± 270 0.4 ND c
6c graphic file with name nihms-1843423-t0014.jpg 709 ± 211 0.29 ND c
6d graphic file with name nihms-1843423-t0015.jpg 1387 ± 262 0.23 ND c
6e graphic file with name nihms-1843423-t0016.jpg 1195 ± 250 0.33 ND c
6f graphic file with name nihms-1843423-t0017.jpg 1446 ± 599 0.32 ND c
6g graphic file with name nihms-1843423-t0018.jpg 1210 ± 267 0.3 ND c
6h graphic file with name nihms-1843423-t0019.jpg >2000 N/A ND c
6i graphic file with name nihms-1843423-t0020.jpg >2000 N/A ND c
6j graphic file with name nihms-1843423-t0021.jpg >2000 N/A ND c
a

Values are the average ± standard deviation of three replicates.

b

LE represents Ligand Efficiency.

c

Tm shift not determined/tested by DSF assay.