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Subcellular localization and in vivo functional identification of TcS. (A) Subcellular localization of TcS in tobacco leaves. Bars = 20 μm. (B) LC–MS fragments of theacrine in the control and TcS transgenic tobacco treated leaves. Injection of 1,3,7-trimethyluric acid into T0 generation TcS transgenic tobacco leaves, transgenic tobacco with empty vector was used in the control group.
