FIG. 4.

Complement consumption by V. anguillarum evaluated by a reduction in complement-mediated hemolysis of RaRBC. RaRBC were incubated in rainbow trout NS as a control (□) or with NS pretreated with smooth V. anguillarum serogroup O1 ATCC 43305 with (○) or without (•) Ab coating, rough V. anguillarum serogroup O1 840606-2/5 without Ab coating (×), V. anguillarum serogroup O2a UB 258/91 with (▵) or without (▴) Ab coating, and V. anguillarum serogroup O2a UB 258/91 grown in glucose-enriched medium and with (◊) or without (⧫) Ab coating. Values shown are means ± standard deviations (n = 4).