Figure 5.

Peroxidase activity of Fe²⁺‐Bisphosphonate Nanoparticles. a,b) Peroxidase activity detection of extracellular Fe²⁺‐Bisphosphonate Nanoparticles catalysts. The chromogen used for (a) was TMB: Tetramethylbenzidine and (b) was DAB: Diaminobenzidine. c) ROS detection of macrophages engulfment of erythrocytes following with zoledronate (BPS) or deferoxamine (DMF) by flow cytometric. d) ROS generation of the CT26 tumor bearing mice in the treatment of bisphosphonates and R300 at Day 3 (n = 8 per group). Scale bars, 100 µm. The RBC represents erythrocyte. Data are shown as the mean ± SD. ^NSp > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. One‐way ANOVA for group comparisons (d).