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. 2022 Sep 12;32(17):3862–3870.e6. doi: 10.1016/j.cub.2022.07.029

Figure 3.

Figure 3

Pathogenic mutations of KIF1C impair force generation

(A) Stills and kymograph from an optical trapping experiment showing processive bead transport by KIF1CP176L-GFP after release of the motor from the trap (bead indicated with orange arrow in stills, indicated time relative to trap release indicated by yellow arrow in kymograph).

(B) Representative traces from optical trapping experiment with KIF1C-GFP wild type and pathogenic mutants P176L and R169W. Dashed lines indicate 8-nm displacement intervals, which are narrower at the low trap stiffness used for mutants.

(C) Histograms of peak forces determined for events lasting longer than 200 ms that exceed 0.5 pN. Mean ± SEM and number of force events are indicated. Groups are significantly different with p < 0.0001 (Kruskal-Wallis test, with Sidak correction).