FIG. 3.
A monoclonal T-cell hybridoma (18-15.18) responds to an antigen(s) from rough strains in a dose-dependent manner. Hybridoma 18-15.18 produces more IL-2 in the presence of LPS mutant strains than in the wild-type LT2 strain. The response increases as LPS length decreases. ConA-elicited macrophages (105/well) were used as APC and combined in a 96-well format with 105 T-cell hybridoma 18-15.18 cells and 107 HKST organisms per well for 24 h. The IL-2 response, recorded in counts per minute (CPM), is expressed as the means ± SD (error bars) for triplicate wells.