FIG. 4.
T-cell hybridoma 18-15.18 responds to live rough LPS mutant bacteria better than to live smooth wild-type bacteria in a dose-dependent manner. ConA-elicited macrophages (105/well) were incubated with live, PBS-washed S. typhimurium bacteria in various concentrations for 2 h. The extracellular bacteria were then removed, and the macrophages were fixed with glutaraldehyde. The T-cell hybridoma was added (105 cells/well) and incubated for 24 h. The IL-2 production in the supernatants was measured. The IL-2 response, recorded in counts per minute (CPM), is expressed as the means ± SD (error bars) for triplicate wells.