Table 3.
Plasma ALT, AST, T4, and T3 (mean ± SE) levels in rainbow trout fed diets continuing 0–500 mg/kg HME for 63 days and subsequently exposed to thermal stress (16–22°C) for 7 days (n = 6).
| HME (mg/kg) | ALT (U/L) | AST (U/L) | T4 (μg/mL) | T3 (ng/mL) | |
|---|---|---|---|---|---|
| Basal | 0 | 23.1 ± 2.72 | 242 ± 15.9 | 16.3 ± 0.54 | 8.32 ± 0.51 |
| 100 | 16.5 ± 1.60 | 239 ± 18.4 | 16.1 ± 0.53 | 7.82 ± 0.50 | |
| 250 | 12.3 ± 1.36 | 172 ± 9.94 | 17.8 ± 0.64 | 7.97 ± 0.61 | |
| 500 | 15.2 ± 2.00 | 233 ± 16.7 | 17.1 ± 0.63 | 8.32 ± 0.59 | |
| Stressed | 0 | 54.8 ± 3.50 | 564 ± 23.6 | 11.6 ± 0.34 | 2.63 ± 0.27 |
| 100 | 46.6 ± 2.21 | 559 ± 28.0 | 11.6 ± 0.30 | 2.88 ± 0.24 | |
| 250 | 36.6 ± 2.32 | 543 ± 24.3 | 12.1 ± 0.35 | 2.90 ± 0.31 | |
| 500 | 40.4 ± 2.42 | 534 ± 14.7 | 12.4 ± 0.42 | 2.62 ± 0.31 | |
| P-value | |||||
| HME | 0.001 > 0b, 100ab, 250a, 500ab | 0.106 | 0.075 | 0.992 | |
| Stress | 0.001>; Basal < Stressed | 0.001>; Basal < Stressed | 0.001>; Basal > Stressed | 0.001>; Basal > Stressed | |
| Interaction | 0.326 | 0.343 | 0.577 | 0.741 | |
The superscript letters indicate the significant differences among the HME concentrations.