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. 2022 Oct 19;7(43):38752–38765. doi: 10.1021/acsomega.2c04368

Figure 4.

Figure 4

Microsomal metabolism of LASSBio-1773 (3) (10 μM) and its hydrolysis metabolite M4, formed in the presence and absence of NADPH. IS, internal standard (biphenyl l-4-carboxylate methyl, 10 μM). (A) Incubation at 0 min, compound 3, Rt = 7.4 min; (B) 20 min incubation time in the presence of NADPH, biotransformation in M4 (Rt = 4.1 min); (C) 20 min incubation time in the absence of NADPH, biotransformation in M4 (Rt = 4.1 min); (D) compound 3, HPLC-MS analysis, m/z 379 [M + H]+; (E) HPLC-MS analysis M4, m/z 365 [M + H]+, formed in the presence of NADPH; (F) HPLC-MS analysis M4, m/z 365 [M + H]+, formed in the absence of NADPH.