Table 2. Permeability Coefficient of N-sulfonylhydrazone Prototypes (1–4) and Quality Control Standards Determined by the PAMPA-BBB Assay.

compounds	literature permeability (10–6 cm/s)a	experimental permeability (10–6 cm/s)	classificationc	pKab
atenolol	0.8	0.10 ± 0.21	BBB–
caffeine	1.3	0.77 ± 0.58	BBB–
enoxacin	0.9	0.57 ± 0.12	BBB–
hydrocortisone	1.9	1.26 ± 0.78	BBB–
ofloxacin	0.8	0.29 ± 0.43	BBB–
testosterone	17	19.9 ± 0.53	BBB+
verapamil	16	16.3 ± 1.74	BBB+
LASSBio-1771 (1)		0.43 ± 0.54	BBB–	6.35
LASSBio-1772 (2)		4.18 ± 0.03	BBB+	ND
LASSBio-1773 (3)		0.45 ± 0.63	BBB–	6.35
LASSBio-1774 (4)		9.01 ± 0.09	BBB+	ND

^aDi et al.²⁶

^bZapata-Sudo et al.¹⁶

^cPe values <1.57 were classified as nonpermeable (BBB−), Pe values >3.86 were correlated with brain penetration ability (BBB+). ND, not determined.