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Immunoblot analysis of S. mansoni soluble extracts and ES products. Extracts of adult male schistosomes (lane 1), adult females (lane 2), mixed-sex cercariae (lane 3), and ES products from mixed-sex adults (lane 4) were separated by SDS-PAGE, transferred to nitrocellulose, and probed with anti-bSmCL1 serum. Replicate filters probed with control (preimmunization) serum showed no reactivity (data not shown).
