FIG. 6.

Immunolocalization of native SmCL1 in adult schistosomes. Longitudinal sections of male worms were probed with preimmunization (A; scale bar = 5 μm) or rabbit anti-bSmCL1 (B; scale bar = 10 μm) serum followed by labeling with anti-rabbit antibody-fluorescein conjugate. No specific labeling was observed with preimmunization serum (A), but intense labeling was observed in the tegument, particularly on the ventral surface, with anti-bSmCL1 (B). Labeling was also observed with anti-bSmCL1 in the gastrodermal cells lining the gut, as shown in the transverse sections of female adult worms (C; scale bar = 2.5 μm). VT, ventral tegument; DT, doral tegument; P, parenchyma; LU, gut lumen; GA, gastrodermis.