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. 2022 Oct 25;55(5):119–128. doi: 10.1267/ahc.22-00075

Fig. 5.

Fig. 5.

Control experiments for MB probes. Paraffin-embedded mouse uterus tissue was used for ISH. Uterine sections were hybridized with complementary MB probes for 28S rRNA (green) (A). Adjacent sections were hybridized with homologous (B) and complementary sequences in the presence of excess amounts of unlabeled complementary (C) and unlabeled homologous oligo-DNAs for 28S rRNA (D). Adjacent section was hybridized with the complementary oligo-DNA for 28S rRNA in the presence of an excess amount of unrelated unlabeled oligo-DNA (E). Tissue was treated with RNase prior to hybridization with MB probe for 28S rRNA (F). Uterine sections were hybridized with complementary oligo-DNA probe (G) homologous (H) and complementary sequences in the presence of excess amounts of unlabeled complementary (I) and unlabeled homologous oligo-DNAs (J) for 28S rRNA. Uterine sections were hybridized with the complementary 28S rRNA in the presence of an excess amount of unrelated unlabeled oligo-DNA (K). Tissue was treated with RNase prior to hybridization with complementary oligo-DNA probes for 28S rRNA (L). Magnification ×400. Bar = 20 μm.