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. 2022 Oct 20;61(21):2295–2302. doi: 10.1021/acs.biochem.2c00482

Figure 2.

Figure 2

Effect of LC3B-115 on ATG4b activity. (a) Schematic illustration of the mass spectrometry-based assay to monitor the cleavage of pro-LC3B to LC3B-I in the presence of LC3B-115. (b) The cleavage of 1 μM pro-LC3B by 50 nM ATG4b in the presence of increasing concentrations of LC3B-115 was monitored by mass spectrometry. LC3B-115 inhibited the cleavage with an IC50 ∼15 nM. (c) Scheme of the ATG4b-mediated cleavage of peptide substrate-33. (d) Steady-state kinetic measurement of ATG4b-mediated cleavage of the fluorescent peptide substrate-33 in the presence and absence of 50 μM LC3B-115.