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Evaluation of binding between LC3B and ATG4b truncations by SPR. (a) ATG4b truncations and their binding affinities to LC3B-I and LC3B-115. (b) Equilibrium binding curves for immobilized biotin-LC3B-I binding to ATG4b-WT, ATG4b-382, ATG4b-366, and ATG4b-354. (c) Equilibrium binding curves for immobilized biotin-LC3B-115 binding to ATG4b-WT, ATG4b-382, ATG4b-366, and ATG4b-354.
