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. 2022 Oct 20;61(21):2295–2302. doi: 10.1021/acs.biochem.2c00482

Figure 4.

Figure 4

Steady-state kinetic analysis of ATG4b constructs. (a) Kinetic parameters for the cleavage of the FRET-LC3B substrate (CFP-LC3B-YFP) and peptide substrate-33 by truncation constructs of ATG4b. (b) Schematic illustration of the FRET assay using the CFP-LC3B-YFP substrate. (c) Turnover of substrate CFP-LC3B-YFP by ATG4b-WT, ATG4b-382, ATG4b-366, and ATG4b-354. (d) Turnover of peptide substrate-33 by ATG4b-WT, ATG4b-382, ATG4b-366, and ATG4b-354. The linear portions of initial rates of product formation were used to determine the kinetic parameters.