FIG. 8.
Immunoblot analysis of four mouse sera obtained 14 days after nasal inoculation with 107 inclusion-forming units of C. pneumoniae. (A) C. pneumoniae EB proteins were solubilized in SDS sample buffer, boiled, separated by SDS-PAGE, and subjected to immunoblot analyses with the four sera. (B) C. pneumoniae EB proteins were suspended in SDS sample buffer without boiling and subjected to immunoblotting with the same four sera as used in panel A. (C) C. pneumoniae OMC proteins were solubilized in SDS sample buffer. The boiled (lane 1) and unboiled (lanes 2 and 3) proteins were subjected to immunoblotting. Lanes 1 and 2 were incubated with serum from mouse 2 (as in Fig. 8A and B, lanes 2), whereas lane 3 was incubated with PAbOmp4.