Upload additional files	DRR-20201001/form/17102020_SB_Cannabis_Reference_Genome_revised (1).docx
Reviewer name and names of any other individual's who aided in reviewer	Wei Zhao
Do you understand and agree to our policy of having open and named reviews, and having your review included with the published papers. (If no, please inform the editor that you cannot review this manuscript.)	Yes
Is the language of sufficient quality?	Yes
Please add additional comments on language quality to clarify if needed
Are all data available and do they match the descriptions in the paper?	No
Additional Comments	The BioProject PRJNA667278 is not accessible.
Are the data and metadata consistent with relevant minimum information or reporting standards? See GigaDB checklists for examples <a href="http://gigadb.org/site/guide" target="_blank">http://gigadb.org/site/guide</a>	Yes
Additional Comments
Is the data acquisition clear, complete and methodologically sound?	Yes
Additional Comments
Is there sufficient detail in the methods and data-processing steps to allow reproduction?	Yes
Additional Comments
Is there sufficient data validation and statistical analyses of data quality?	No
Additional Comments	The size of the final genome assembly is significantly larger than the estimated size, which is indicative of redundancy. I would suggest removing the potential haplotype redundancy further. I would also suggest a k-mer analysis to validate the genome size. For a chromosomal assembly, the ratio of properly paired reads is lower than expected.
Is the validation suitable for this type of data?	Yes
Additional Comments
Is there sufficient information for others to reuse this dataset or integrate it with other data?	Yes
Additional Comments
Any Additional Overall Comments to the Author
Recommendation	Major Revision