Table 3.

Estimation of genome size and heterozygosity of L. d. dumerilii by k-mer analysis.

k	Total number of k-mers	Minimum coverage (X)	Number of erroneous k-mers	Homozygous peak	Estimated genome size (Gb)	Estimated heterozygosity (%)
17	112,401,363,509	9	1,418,748,938	45	2.47	1.10
19	110,136,516,133	8	2,588,664,358	43	2.50	1.23
21	107,871,808,889	7	3,023,604,282	42	2.50	1.24
23	105,607,392,491	7	3,286,834,146	40	2.56	1.22
25	103,343,108,760	7	3,501,481,190	39	2.56	1.19
27	101,078,882,097	7	3,689,197,189	38	2.56	1.16
29	98,815,880,190	6	3,839,002,752	37	2.57	1.14
31	96,552,885,503	6	3,986,778,359	36	2.57	1.11

Note: k-mer frequency distributions were generated by Jellyfish (v2.2.6) using 131 Gb clean sequences as input. Minimum coverage was the coverage depth value of the first trough in k-mer frequency distribution. k-mers with coverage depth less than the minimum coverage were regarded as erroneous k-mers. Estimated genome size was calculated as (Total number of k-mers – Number of erroneous k-mers) / Homozygous peak.