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Optimized gradient for LC-MS/MS analysis for the quantification of cysteamine in plasma.
| Time | % Eluent A | % Eluent B | Flow |
|---|---|---|---|
| 0.0 | 99 | 1 | 0.4 mL/min |
| 6.0 | 80 | 20 | 0.4 mL/min |
| 6.5 | 0 | 100 | 0.4 mL/min |
| 8.5 | 0 | 100 | 0.4 mL/min |
| 8.6 | 99 | 1 | 0.4 mL/min |
| 14 | 99 | 1 | 0.4 mL/min |
