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. 2022 Nov 3;18:388. doi: 10.1186/s12917-022-03475-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Alkaline phosphatase activity and Alizarin Red S concentration of osteogenic induced and control cells. Bar plot showing alkaline phosphatase (ALP) activity (A) and Alizarin Red S concentration (B) as a measurement of calcium deposits of equine bone marrow (BM)- and adipose tissue (AT)-derived mesenchymal stromal cells (MSC) and dermal fibroblasts (DF) (biological negative control) in five different age groups after respectively 7 or 21 days of culture in either osteogenic induction medium or expansion medium (non-induced control) (n = 4 horses per cell type per age group per medium). Age groups within the same osteogenic induced cell type not labeled with the same letter are significantly different from each other with regards to calcium deposition (p < 0.05). Error bars indicate standard deviation. ^†N: Newborn, Y: Yearling, A: Adult, M: Middle-aged, G: Geriatric