Table 3.
Overview of TaqMan primer–probe sets used in RT-qPCR reactions
| Gene ID | Gene name(s) | ThermoFisher Assay ID |
|---|---|---|
| ABI3BP | ABI family member 3 binding protein | Ec06625599_m1 |
| ACAN | Aggrecan core protein | Ec03469667_m1 |
| ALPL | Alkaline phosphatase | Ec06625502_g1 |
| AQP1 | Aquaporin | Ec06625425_m1 |
| AREG | Amphiregulin | Ec06992855_m1 |
| BARD1 | BRCA1-associated RING domain 1 | Ec07061151_m1 |
| BAX | BCL2 associated X apoptosis regulator | Ec07016716_s1 |
| BCL2 | B-cell lymphoma 2 | Ec07005800_g1 |
| BGN | Biglycan | Ec03467971_m1 |
| BMP2 | Bone morphogenic factor 2 | Ec06974239_m1 |
| BMP3 | Bone morphogenic factor 3 | Ec07037656_m1 |
| BMP4 | Bone morphogenic factor 4 | Ec03470252_s1 |
| BMP6 | Bone morphogenic factor 6 | Ec03469925_m1 |
| BMP7 | Bone morphogenic factor 7 | Ec04320876_m1 |
| BRCA1 | Breast cancer type 1 | Ec07017862_s1 |
| CASP3 | Caspase 3 | Ec03470391_m1 |
| CASP8 | Caspase 8 | Ec06959413_m1 |
| CAVIN1 | Caveolae associated protein 1 | Ec07036873_m1 |
| CCND1 | Cyclin D1 | Ec07036996_m1 |
| CDKN1A | p21, Cyclin-dependent kinase inhibitor 1A | Ec06955195_m1 |
| CHADL | Chondroadherin | Ec03470206_m1 |
| CLU | Clusterin | Ec03468575_m1 |
| COL1A1 | Collagen type 1 alpha 1 | Ec03469676_m1 |
| COL2A1 | Collagen type 2 alpha 1 | Ec03467411_m1 |
| COL4A1 | Collagen type 4 alpha 1 | Ec06943950_m1 |
| COL5A3 | Collagen type 5 alpha 3 | Ec06999559_g1 |
| COL9A2 | Collagen type 9 alpha 2 | Ec03470083_m1 |
| COL11A1 | Collagen type 11 alpha 1 | Ec07051918_m1 |
| COL12A1 | Collagen type 12 alpha 1 | Ec03469523_m1 |
| COMP | Cartilage oligomeric matrix protein | Ec03468062_m1 |
| CRTAC1 | Cartilage acidic protein –CEP-68 | Ec07040335_g1 |
| CRYAB | Crystalline-alpha B | Ec06997901_m1 |
| CSF2 | Colony stimulating factor 2 | Ec03468208_m1 |
| CTGF | Connective tissue growth factor | Ec06625777_gH |
| CTNNB1 | Beta-catenin | Ec00991819_m1 |
| DCN | Decorin | Ec03468474_m1 |
| DMP1 | Dentin matrix acidic phosphoprotein 1 | Ec06992382_m1 |
| EBF2 | Early B-cell factor 2 | Ec06966528_m1 |
| EPGN | Epithelial mitogen | Ec06992859_m1 |
| FGF1 | Fibroblast growth factor 1 | Ec01092738_m1 |
| FGF5 | Fibroblast growth factor 5 | Ec04656774_m1 |
| FGF18 | Fibroblast growth factor 18 | Ec03248217_g1 |
| FGFR3 | Fibroblast growth factor receptor 3 | Ec03470545_m1 |
| FN1 | Fibronectin 1 | Ec03470760_m1 |
| GDF6 | Growth differentiation factor 6 | Ec07097112_m1 |
| GLB1 | Beta-galactosidase | Ec06954363_m1 |
| GLI3 | GLI family zinc finger 3 | Ec06625512_m1 |
| HAPLN1 | Hyaluronan and proteoglycan link protein 1 | Ec03468716_m1 |
| HDGF | Hepatoma-derived growth factor | Ec07037751_m1 |
| HGF | Hepatocyte growth factor | Ec07000054_m1 |
| IBSP | Integrin Binding Sialoprotein | Ec06625402_m1 |
| IGF1 | Insulin-like growth factor 1 | Ec03468689_m1 |
| IGFBP5 | Insulin-like growth factor binding protein 5 | Ec03470296_m1 |
| LOC100146-270 | p16, Cyclin-dependent kinase 4 inhibitor B | Ec07037471_mH |
| LOC100146-589 | Osteocalcin | Ec07103628_m1 |
| MATN1 | Matrilin-1 | Ec06963427_m1 |
| MEPE | Matrix extracellular phosphoglycoprotein | Ec06992377_m1 |
| MET | MET proto-oncogene, receptor tyrosine kinase | Ec02622441_m1 |
| MIA | Cartilage-derived retinoic acid-sensitive protein | Ec03469434_m1 |
| MMP3 | Matrix metalloproteinase 3 | Ec03468676_m1 |
| MMP13 | Matrix metalloproteinase 13 | Ec03467796_m1 |
| MYC | c-myc | Ec07007511_m1 |
| NODAL | Nodal growth differentiation factor | Ec07036659_m1 |
| OMD | Osteomodulin | Ec06625496_m1 |
| PCNA | Proliferating cell nuclear antigen | Ec06974312_m1 |
| PDGFD | Platelet-derived growth factor subunit D | Ec06997714_m1 |
| PHB | Prohibitin | Ec07055990_m1 |
| PHEX | Phosphate regulating endopeptidase homolog, X-linked | Ec07034292_s1 |
| PTCH2 | Patched 2 | Ec06625424_g1 |
| RUNX2 | Runt-related transcription factor 2 | Ec03469741_m1 |
| RUNX3 | Runt-related transcription factor 3 | Ec06625430_g1 |
| S100A1 | S100 calcium binding protein A1 | Ec03470173_g1 |
| SMOC1 | SPARC related modular calcium binding | Ec06978758_m1 |
| SMPD3 | Sphingomyelin phosphodiesterase 3 | Ec06625668_m1 |
| SNAI2 | Snail family transcriptional repressor 2 | Ec06625397_m1 |
| SOST | Scherostin | Ec07036868_m1 |
| SOX5 | SRY-box transcription factor 5 | Ec01552798_m1 |
| SOX9 | SRY-box transcription factor 9 | Ec03469763_s1 |
| SP7 | Osterix | Ec06625770_g1 |
| SPARC | Osteonectin | Ec03818202_m1 |
| SPP1 | Osteopontin, Secreted phosphoprotein 1 | Ec06992376_g1 |
| TBX3 | T-box 3 | Ec07003300_m1 |
| TERT | Telomerase reverse transcriptase | Ec06972692_m1 |
| TGFA | Transforming growth factor alpha | Ec06949183_m1 |
| TGFB1 | Transforming growth factor beta 1 | Ec06625477_m1 |
| TGFB2 | Transforming growth factor beta 2 | Ec07074189_g1 |
| TGFB3 | Transforming growth factor beta 3 | Ec00682163_m1 |
| TIMP2 | Metallopeptidase inhibitor 2 | Ec03470558_m1 |
| TNF | Tumor necrosis factor | Ec03467871_m1 |
| TNFRSF11B | Osteoprotegerin | Ec07007303_m1 |
| TNFSF11 | RANKL, TNF superfamily member 11 | Ec06625532_m1 |
| TP53 | Tumor protein 53 | Ec03470648_m1 |
| VEGFA | Vascular endothelial growth factor A | Ec03467879_m1 |
| B2M | Beta-2-microblobulin | Ec03468699_m1 |
| GUSB | Beta-glucoronidase | Ec03470630_m1 |
| RPLP0 | Ribosomal protein lateral stalk subunit P0 | Ec04947733_g1 |