Fig. 5.

Apoptotic cell death induced by PDE10A inhibitors is mediated by PKA and PKG. A OV-90 cells pre-treated with the PKG inhibitor, KT-5823 (left), for 30 min or the PKA inhibitor, H-89 (right), for 10 min blocks Pf-2545920 activation of PKG and PKA, respectively, in a dose-dependent manner (Pf-2545920 treatment for 1 h). GAPDH was used as a loading control. B OV-90 cells pre-treated with the PKG inhibitor, KT-5823 (left), and the PKA inhibitor, H-89 (right), blocks Pf-2545920 induction of apoptosis (KT-5823 treatment for 30 min, H-89 10 min, Pf-2545920 treatment for 6 h). GAPDH was used as a loading control. C-D OV-90 and SKOV3 cells pre-treated with the PKG inhibitor, KT-5823 (left), for 30 min or the PKA inhibitor, H-89 (right), for 10 min blocks MCI-030 induction of apoptosis (MCI-030 treatment for 24 h). GAPDH was used as a loading control