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. 2022 Oct 20;13:1016914. doi: 10.3389/fimmu.2022.1016914

Figure 1.

Figure 1

CEACAM expression on CD14+16- classical monocytes. (A) Gating strategy for identification of CEACAM+CD14+16- classical monocytes using flow cytometry. (B) Giemsa staining for CEACAM-negative (CEACAM-) and CEACAM-positive (CEACAM+) classical monocytes. Sorted CEACAM- and CEACAM+ cells were stained with May-Grünwald Giemsa after cytospin centrifugation. Scale bar represents 20 µm. (C, D) CEACAM staining for classical monocytes identified using flow cytometry. Dot plots show CEACAM staining gated on CD14+16- cells from healthy controls (HCs) (n = 34) and patients with rheumatoid arthritis (RA) (n = 20), limited cutaneous systemic sclerosis (lcSSc) (n=12), and diffuse cutaneous systemic sclerosis (dcSSc) (n = 18) analyzed using flow cytometry. Representative data for each group are shown (C). The proportion of CEACAM+ cells in classical monocytes is plotted (D). (E) The proportion of CEACAM+CD14+16- monocytes was compared between patients with SSc within 2 years after diagnosis (< 2 years) and those with SSc over 2 years after diagnosis (> 2 years). P values were calculated using one-way ANOVA with Tukey’s-multiple comparisons testing between all groups (D) and Mann–Whitney U test (E). *P < 0.05, **P < 0.01.