Table 1.
Demographic and sequencing information on the studies with immune repertoire data from both B and T cells used for analysis.
| Author(Year) | Sequencing method, Data processing | N | Median age (range) | Gender (% female) | B/T average clonotype count (SD) | |
|---|---|---|---|---|---|---|
|
Healthy
(N = 152) |
Rubelt et al. (2016) |
Immune repertoire sequencing of FACS-sorted B/T cells (RNA), VDJPipe, pRESTO, IMGT/HighV-QUEST |
10 | NA (22-27) | NA | 20852 (13164) 24791 (8908) |
| Schultheiss et al. (2021) |
Bulk immune repertoire sequencing (gDNA), Mixcr |
57 | 50 (23-86) | 60 | 1610 (1300) 7314 (3667) |
|
| Schultheiss et al. (2020) |
Bulk immune repertoire sequencing (gDNA) Mixcr |
37 | NA | NA | 2311 (1298) 5818 (3058) |
|
| Wen et al. (2020) |
10x chromium single-cell V(D)J enrichment (RNA) Cell Ranger vdj pipeline |
5 | 55 (30-80) | 60 | 951 (352) 5449 (1745) |
|
| Joseph et al. (2022) |
immunoPETE protocol from Roche Sequencing (gDNA) In-house pipeline |
43 | 43 (25-71) | 44 | 8394 (5643) 23412 (12193) |
|
|
DA
(N = 133) |
Schultheiss et al. (2022) |
Bulk immune repertoire sequencing (gDNA), Mixcr |
54 (AIH) |
54 (20-79) | 80 | 1649 (1309) 6863 (5298) |
| Schultheiss et al. (2021) |
Bulk immune repertoire sequencing (gDNA), Mixcr |
37 (COVID) | NA (20-79) | 32 | 3980 (2556) 9197 (6460) |
|
| Wen et al. (2020) |
10x chromium single-cell V(D)J enrichment (RNA) Cell Ranger vdj pipeline |
10 (COVID) | 58 (30-80) | 50 | 999 (587) 2250 (1282) |
|
| Joseph et al. (2022) |
immunoPETE protocol from Roche Sequencing (gDNA) In-house pipeline |
32 (COVID) |
60 (28-88) | 38 | 10145 (10743) 22059 (15431) |
DA, disease-associated.
AIH, autoimmune hepatitis.
NA, not available.