REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-Miranda (1:2000)	Betschinger et al.71	N/A
Mouse monoclonal anti-phospho-Histone H3 (pH3) Ser 10 (1:1000)	Cell Signalling	Cat# 9706; RRID: AB_331748
Rabbit anti-phospho-Histone H3 (pH3) Ser10 (1:2000)	Merck Millipore	Cat# 06-570; RRID: AB_310177
Rabbit anti-aPKC (c20, 1:2000)	Santa Cruz Biotechnology	Cat# sc-208; RRID: AB_2168668
Mouse anti-Crumbs (1:50)	Developmental Studies Hybridoma Bank	Cat# cq4; RRID: AB_528181
Rabbit anti-Yurt (1:2000)	Biehler et al.36	RRID: AB_2568494
Mouse anti-α-Tubulin (DM1A, 1:10 000)	Santa Cruz Biotechnology	Cat# sc-32293; RRID: AB_628412
Alexa Fluor 568-conjugated goat anti-rabbit	Thermo Fisher Scientific	Cat# A-11036; RRID: AB_10563566
Alexa Fluor 647-conjugated goat anti-rabbit	Thermo Fisher Scientific	Cat# A-31573; RRID: AB_2536183
Alexa Fluor 647-conjugated goat anti-mouse	Thermo Fisher Scientific	Cat# A-31571; RRID: AB_162542
Chemicals, peptides, and recombinant proteins
Insulin solution from bovine pancreas	Sigma-Aldrich	Cat# I0516
Schneider’s Insect Medium	Sigma-Aldrich	Cat# S0146
Phalloidin-FITC	Sigma-Aldrich	Cat# P5282
Phalloidin-TRITC	Sigma-Aldrich	Cat# P1951
Phalloidin-CruzFluor647	Santa Cruz Biotechnology	Cat# sc-363797
Cell Mask Orange Plasma Membrane Stain	Thermo Fisher	Cat# C10045
Latrunculin A	Sigma-Aldrich	Cat# 428021
Colchicine	Sigma-Aldrich	Cat# C9754
Binucleine-2	Sigma-Aldrich	Cat# B1186
Thrombin	Sigma-Aldrich	Cat# T7513
Glucose	Sigma-Aldrich	Cat# G7021
Fibrinogen	Sigma-Aldrich	Cat# F8630
Experimental models: Organisms/strains
D. melanogaster: Par6::mCherry:;; UAS-Par6mCherry	Doerflinger et al.72	N/A
D. melanogaster: GFP::aPKC:;aPKC>GFP::aPKC;	Chen et al.73	N/A
D. melanogaster: Lgl::mCherry:;lgl>lgl::mCherry;	Dong et al.15	N/A
D. melanogaster: E-cad::GFP:;shg>shg::GFP;	Huang et al.74	N/A
D. melanogaster: E-cad::mKate2:;shg>shg::3xmKate2/CyoGFP;	Pinheiro et al.75	N/A
D. melanogaster: E-cad::3xGFP:;shg>shg::3xGFP/Cyo;	Pinheiro et al.75	N/A
D. melanogaster: Zip::YFP:;PBac{544.SVS-1}zipCPTI100036/SM6a;	Kyoto Stock Center	RRID: DGGR_115082
D. melanogaster: Sqh::mKate2:;P{w+, sqh>sqh::3xmKate2}/CyO;	Pinheiro et al.75	N/A
D. melanogaster: Sqh::mKate2:;;P{w+, sqh>sqh::3xmKate2}/TM6B,Tb	Pinheiro et al.75	N/A
D. melanogaster: H2A::RFP:;;P{w+, his2Av>his2Av::mRFP}/MKRS	Pandey et al.76	N/A
D. melanogaster: mCherry::Cdc42:;;Sqh-mCherry::Cdc42	Bloomington Drosophila Stock Center	RRID: BDSC_42237
D. melanogaster: UAS-LARIAT:;P{w+, UASt>VHH(GFP)::SNAP::CRY2(PHR)::P2A::CIBN::MP}/CyO;	Lee et al.30	N/A
D. melanogaster: UAS-LARIAT:;;P{w+, UASt>VHH(GFP)::SNAP::CRY2(PHR)::P2A::CIBN::MP}/TM6B,Tb	Qin et al.77	N/A
D. melanogaster: PatJ::CIBN::GFPpm:;P{w+, UASp>PatJ::CIBN::pmGFP}/CyO;	Krueger et al.40	N/A
D. melanogaster: RhoGEF::CRY2::mCherry:;;P{w+, UASp>RhoGEF2::CRY2::mCherry}/TM3,Ser	Izquierdo et al.78	N/A
D. melanogaster: RhoGEF::CRY2:;;P{w+, UASp> RhoGEF2::CRY2}/TM3,Ser	Izquierdo et al.78	N/A
D. melanogaster: mCherry:;;P{y+ v+, UAS>mCherry.VALIUM10}attP2	Bloomington Drosophila Stock Center	RRID: BDSC_35787
D. melanogaster: Tum RNAi:;;P{y+ v+, UAS>tum RNAi.VALIUM10}attP2	Bloomington Drosophila Stock Center	RRID: BDSC_28982
D. melanogaster: SqhE20E21:;;P{w+, UASp> UASp>SqhE20E21}	Bloomington Drosophila Stock Center	RRID: BDSC_64411
D. melanogaster: SqhA20A21:;;P{w+, UASp> UASp>SqhA20A21}	Bloomington Drosophila Stock Center	RRID: BDSC_64114
D. melanogaster: tj>Gal4:;P{w+=GawB}NP1624/CyO;	Kyoto Stock Center	RRID: DGGR_104055
D. melanogaster: GR1>Gal4:;;P{w+=GawB}GR1/TM6B, Tb	Gupta and Schüpbach79	N/A
D. melanogaster: pnt>Gal4:	Vienna Drosophila Resource Center	VDRC_VT212057 (discarded)
D. melanogaster: Gal80ts:;;P{w+, tubP>Gal80ts}	Bloomington Drosophila Stock Center	RRID: BDSC_7018
D. melanogaster: nlsRFP hs-Flp FRT19A: P{w+, ubi>nlsRFP} w∗P{ry+, hs>Flp}12 P{ry+, neoFRT}19A;;	Bloomington Drosophila Stock Center	RRID: BDSC_31418
D. melanogaster: hs-Flp Gal80 FRT19A: P{ry+, hs>Flp}1 P{w+, tubP>Gal80} w∗P{ry+, neoFRT}19A;;	Bloomington Drosophila Stock Center	RRID: BDSC_5133
D. melanogaster: aPKC-:;P{lacW}aPKCk06403/CyO;	Wodarz et al.80	N/A
D. melanogaster: aPKCas4:;aPKCas4;	Hannaford et al.35	N/A
D. melanogaster: aPKCas4::mScarlet:;aPKCas4::mScarlet;	This paper	N/A
Software and algorithms
FIJI	Schindelin et al.81	N/A
GraphPad Prism 6 and 8	GraphPad Software (La Jolla, CA, USA)	N/A
Other
Blue light LED bulb (474nm)	SuperBrightLEDs	Cat# MR16-B48SMD
Amber light LED bulb (593nm)	SuperBrightLEDs	Cat# MR16-Y48SMD
Laser Scanning Confocal Microscope TCS SP5 II	Leica Microsystems	N/A
Laser Scanning Confocal Microscope TCS SP8	Leica Microsystems	N/A
Zeiss Axio Imager Z1 microscope	Carl Zeiss	N/A
Zeiss Axio Imager Z1 Apotome microscope	Carl Zeiss	N/A
Zeiss LSM880 Confocal	Carl Zeiss	N/A
Zeiss LSM710 Confocal	Carl Zeiss	N/A
Spinning disk confocal microscope Andor Revolution XD	Andor Technology	N/A