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. 2022 Oct 20;13:943097. doi: 10.3389/fmicb.2022.943097

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Copyright © 2022 Jin, Zhou, Lan, Li, Yang and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LEfSe results for the egg white and yolk. LEfSe analysis was used with an LDA score > 4.0 to show differential enrichment of the bacterial features as biomarkers between egg yolks and whites in different groups. (A,B) LEfSe shows the differential microbiota between fresh egg yolks and whites. (C,D) LEfSe shows the differential microbiota between egg yolks and whites at 12 embryonic ages. (E) Random forest analysis identifies the key differential genera between egg yolks and whites at different periods.