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. 2022 Nov 3;13:6603. doi: 10.1038/s41467-022-34080-4

Fig. 6. TNF-induced cell death is responsible for systemic inflammation and emergency hematopoiesis in RIPK1 Y383F mice.

Fig. 6

a Flow cytometry and statistical analysis for neutrophils (CD11b+Ly6G+) in the spleen from Ripk1+/+, Ripk1Y383F/Y383F, Tnfr1−/− and Ripk1Y383F/Y383F Tnfr1−/− mice at age of 8 weeks (n = 3). b Immunohistochemical staining of CD11b, Ly6G, and CD45 of liver sections from Ripk1+/+, Ripk1Y383F/Y383F, Tnfr1−/− and Ripk1Y383F/Y383F Tnfr1−/− mice at age of 8 weeks (Scale bar, 100 μm). Flow cytometry and statistical analysis in bone marrow for the indicated hematopoietic populations: LKs, LSKs, HSCs (c) and GMPs, MEPs, CMPs (d) from Ripk1+/+, Ripk1Y383F/Y383F, Tnfr1−/−and Ripk1Y383F/Y383F Tnfr1−/− mice at age of 8 weeks (n = 3). e Ripk1+/+ and Ripk1Y383F/Y383F mice were treated with Nec-1s (3 mg/kg) every one day after 3 weeks old (n = 3). After 8 weeks, neutrophils (CD11b+Ly6G+) in the spleen from the mice were analyzed by flow cytometry. f Flow cytometry and statistical analysis for neutrophils (CD11b+Ly6G+) in the spleen from Ripk1+/+, Ripk1Y383F/Y383F, Ripk3−/−Caspase8−/− and Ripk1Y383F/Y383F Ripk3−/−Caspase8−/− mice at age of 8 weeks (n = 3). g Immunohistochemical staining of CD11b, Ly6G, and CD45 of liver sections from Ripk1+/+, Ripk1Y383F/Y383F, Ripk3−/−Caspase8−/− and Ripk1Y383F/Y383F Ripk3−/−Caspase8−/− mice at age of 8 weeks (Scale bar, 100 μm). h, i Flow cytometry and statistical analysis in bone marrow for the indicated hematopoietic populations: LKs, LSKs, HSCs (h) and GMPs, MEPs, CMPs (i) from Ripk1+/+, Ripk1Y383F/Y383F, Ripk3−/−Caspase8−/− and Ripk1Y383F/Y383F Ripk3−/−Caspase8−/− mice at age of 8 weeks (n = 3). Data are represented as mean ± SEM. Statistical significance was determined using a two-tailed unpaired t test. n.s. p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001. Source data are provided with this paper.