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. 2022 Apr 20;1(4):e36. doi: 10.1002/jex2.36

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© 2022 The Authors. Journal of Extracellular Biology published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Tetraspanin expression analysis of unprocessed aqueous humor samples using the SP‐IRIS analysis. Samples were analysed by the Exoview R100 system. (a) Representative fluorescent images detected by fluorescent‐conjugated antibodies (red: CD63‐AF647, green: CD81‐AF555 and blue: CD9‐ AF488) on Exoview tetraspanin chip immunocapture spots. (a) Total fluorescent particle counts normalized to isotype control IgG on a single capture spot in individual samples. (c,d) Total fluorescent particle counts normalized to isotype control IgG determined by detection antibody on three capture spots in five pediatric disease states. Error bars represent ± one S.E.M