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. 2022 Nov 3;20(11):e3001790. doi: 10.1371/journal.pbio.3001790

Fig 3. Induction of the GTA cluster leads to GTA packaging and lysis.

Fig 3

(A) Long-read (PacBio) sequencing of DNA packaged during GTA production. Inset displays the packaging of the GTA cluster, in gray, and the surrounding DNA. Data are available in S1 Data. (B) Survival of cells bearing high-copy plasmids driving xylose-inducible expression of different genes. Cells were induced with 0.3% xylose and CFUs were enumerated over time. (C) Quantification of released protein content in supernatant during induction of gafYZ over time in strains bearing either Pxyl-gafYZ (black circles) or empty vector (white circles) with 0.3% xylose in PYE (n = 3, error bars indicate SD). * = p-value <0.05 between data of same time point. Data are available in S1 Data. (D) Quantification of released protein content in supernatant (magenta) as wild-type and ΔrogA cells enter stationary phase (n = 3, error bars indicate SD). Optical density of cells is plotted in black. Data are available in S1 Data. CFU, colony-forming unit;, GTA, gene transfer agent.